Saururus chinensis Prevents Estrogen Deficiency-Induced Osteoporosis in Rats: A Metabolomic Study Using UPLC/Q-TOF MS

Saururus chinensis (SC), a traditional medicine, has been used for the treatment of edema, jaundice, gonorrhea, and several inflammatory diseases in China Korea. Our previous studies reported anti-osteoporotic activity SC extract (SCE) ovariectomized (OVX) rats but mechanism this effect was unclear. The aim study to explore SCE elucidate underlying mechanisms using metabolomics approach based on ultra-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC/ESI-Q-TOF MS) analyze metabolic changes. Female Sprague-Dawley (SD) were divided into sham, OVX, groups. Partial least squares-discriminant analysis (PLS-DA) orthogonal partial (OPLS-DA) score plots separated OVX sham groups fairly well. Further, 22 potential biomarkers identified rat models estrogen deficiency-induced osteoporosis, showed regulatory effects three vital pathways associated these biomarkers, namely, glycerophospholipid metabolism, glycosylphosphatidylinositol-anchor biosynthesis, linoleic acid metabolism. reliably confirmed that exerted its by intervening lipid thus preventing osteoporosis. This also promising an evaluation natural medicine efficacy.